Journal: Frontiers in Neuroscience

Article Title: Protecting RNA quality for spatial transcriptomics while improving immunofluorescent staining quality

doi: 10.3389/fnins.2023.1198154

Figure Lengend Snippet: Result summary of tested antibodies in our optimized protocol.

Article Snippet: PSD95 , Excitatory postsynapse , ms , StressMarq , SMC-122D , AB_2300386 , 1:500; 2 μg/mL , No.

Techniques: Marker, Concentration Assay

Journal: IBRO Neuroscience Reports

Article Title: IL-33 relieves nerve injury by mediating microglial polarization in neuromyelitis optica spectrum disorders via the IL-33/ST2 pathway

doi: 10.1016/j.ibneur.2024.07.008

Figure Lengend Snippet: Microglial cells were activated and transformed to the M2 phenotype after treatment with IL-33. (a) Western blot images and the quantification of CD206 and CD40 levels after treatment with IL-33 or NMOD serum. (b) BV2 cells were labeled with Iba-1 and CD68 for immunofluorescence. (c) Expression levels of PSD95 were observed by immunofluorescence after treatment with IL-33 or NMOSD serum. One-way analysis of variance was used for statistical analyses. *p<0.05, **p<0.01, ***p<0.001.

Article Snippet: For protein immunoreactivity in cells, the primary cortical neurons or BV2 cells were fixed with 4 % paraformaldehyde for 30 min at RT and treated with 0.5 % Triton for 10 min. After being washed with PBS, the cells were blocked with 5 % bovine serum albumin (BSA) for 1 h and then incubated with a rabbit anti-PSD95 antibody (1:100 dilution, PA2295, Boster), anti-CD68 antibody (1:200 dilution, 28058–1-AP, Proteintech), anti-IBA1 antibody (1:100 dilution, CY7217, Abways), or anti-ST2 antibody (1:200 dilution, PRS3363, Sigma) at 4 °C overnight.

Techniques: Transformation Assay, Western Blot, Labeling, Immunofluorescence, Expressing